TRAV1-2+ CD8+ T-cells including oligoconal expansions of MAIT cells are enriched in the airways in human tuberculosis


Mucosal-associated invariant T (MAIT) cells typically express a TRAV1-2+ semi-invariant TCRα that enables recognition of bacterial, mycobacterial, and fungal riboflavin metabolites presented by MR1. MAIT cells are associated with immune control of bacterial and mycobacterial infections in murine models. Here, we report that a population of pro-inflammatory TRAV1-2+ CD8+ T cells are present in the airways and lungs of healthy individuals and are enriched in bronchoalveolar fluid of patients with active pulmonary tuberculosis (TB). High-throughput T cell receptor analysis reveals oligoclonal expansions of canonical and donor-unique TRAV1-2+ MAIT-consistent TCRα sequences within this population. Some of these cells demonstrate MR1-restricted mycobacterial reactivity and phenotypes suggestive of MAIT cell identity. These findings demonstrate enrichment of TRAV1-2+ CD8+ T cells with MAIT or MAIT-like features in the airways during active TB and suggest a role for these cells in the human pulmonary immune response to Mycobacterium tuberculosis.

Emily B. Wong
Marielle C. Gold
Erin W. Meermeier
Bongiwe Z. Xulu
Sharon Khuzwayo
Zuri A. Sullivan
Eisa Mahyari
Zoe Rogers
Henrik Kloverpris
Prabhat K. Sharma
Aneta H. Worley
Umesh Lalloo
Prinita Baijnath
Anish Ambaram
Leon Naidoo
Moosa Suleman
Rajhmun Madansein
James E. McLaren
Kristin Ladell
Kelly L. Miners
David A. Price
Samuel M. Behar
Morten Nielsen
Victoria O. Kasprowicz
Alasdair Leslie
William R. Bishai
Thumbi Ndung’u
David M. Lewinsohn
Communications Biology
Publication Date: 
June, 2019
IBN number: